image lab tm software version 5 2 1 (Bio-Rad)
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Image Lab Tm Software Version 5 2 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 36371 article reviews
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Software:Article Title: Neuroactive Steroid–Gut Microbiota Interaction in T2DM Diabetic Encephalopathy Article Snippet: .. The ECL method was used to detect protein bands, and the signals were acquired with a ChemiDoc TM XRS+ system (Bio-Rad, Segrate, Italy) and analyzed with Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Article Title: Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence Article Snippet: The protein bands were visualized using enhanced chemiluminescence (ECL, Amersham ECL Prime Western Blotting Detection Reagent, GE Healthcare, Chicago, IL, USA) and chemiluminescence detection using a ChemiDoc TM MP Imaging System (Bio-Rad, Herakles, CA, USA). .. The band intensity was quantified and normalized to that of β-actin using Article Title: Altered Capacity for H 2 S Production during the Spontaneous Differentiation of Caco-2 Cells to Colonocytes Due to Reciprocal Regulation of CBS and SELENBP1 Article Snippet: .. Thereafter, black PbS spots were detected on the agarose gel using a ChemiDoc TM MP analyzer, and pixel density was determined using Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with Article Title: Diabetic Encephalopathy in a Preclinical Experimental Model of Type 1 Diabetes Mellitus: Observations in Adult Female Rat Article Snippet: After washing, the protein bands were detected on membranes using the ECL method (Bio-Rad, Segrate, Italy). .. ECL signals were acquired with a ChemiDocTM XRS+ system (Bio-Rad, Segrate, Italy) and analysed with Polymerase Chain Reaction:Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Agarose Gel Electrophoresis:Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Article Title: Altered Capacity for H 2 S Production during the Spontaneous Differentiation of Caco-2 Cells to Colonocytes Due to Reciprocal Regulation of CBS and SELENBP1 Article Snippet: .. Thereafter, black PbS spots were detected on the agarose gel using a ChemiDoc TM MP analyzer, and pixel density was determined using Staining:Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Imaging:Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Hood:Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Membrane:Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with Incubation:Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with |
