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image lab tm software version 5 2 1  (Bio-Rad)


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    Bio-Rad image lab tm software version 5 2 1
    Image Lab Tm Software Version 5 2 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+lab+tm+software+version+5+2+1/Image+Lab+Software/pmc12565455-102-80-89
    Average 99 stars, based on 36371 article reviews
    image lab tm software version 5 2 1 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: Neuroactive Steroid–Gut Microbiota Interaction in T2DM Diabetic Encephalopathy
    Article Snippet: .. The ECL method was used to detect protein bands, and the signals were acquired with a ChemiDoc TM XRS+ system (Bio-Rad, Segrate, Italy) and analyzed with Image Lab TM software version 5.2.1 (Bio-Rad, Segrate, Italy). ..

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Three liver tumor organoids were pooled into one, and total RNA was isolated using TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands
    Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Image Lab TM software version 5.2.1 (BioRad, Hercules, CA, USA). ..

    Article Title: Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
    Article Snippet: The protein bands were visualized using enhanced chemiluminescence (ECL, Amersham ECL Prime Western Blotting Detection Reagent, GE Healthcare, Chicago, IL, USA) and chemiluminescence detection using a ChemiDoc TM MP Imaging System (Bio-Rad, Herakles, CA, USA). .. The band intensity was quantified and normalized to that of β-actin using Image Lab TM software Version 5.2.1 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Altered Capacity for H 2 S Production during the Spontaneous Differentiation of Caco-2 Cells to Colonocytes Due to Reciprocal Regulation of CBS and SELENBP1
    Article Snippet: .. Thereafter, black PbS spots were detected on the agarose gel using a ChemiDoc TM MP analyzer, and pixel density was determined using Image Lab TM software, version 5.2.1 (Bio-Rad Laboratories). ..

    Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Total RNA was prepared from liver organoids treated with TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Diabetic Encephalopathy in a Preclinical Experimental Model of Type 1 Diabetes Mellitus: Observations in Adult Female Rat
    Article Snippet: After washing, the protein bands were detected on membranes using the ECL method (Bio-Rad, Segrate, Italy). .. ECL signals were acquired with a ChemiDocTM XRS+ system (Bio-Rad, Segrate, Italy) and analysed with Image Lab TM software version 5.2.1 (Bio-Rad, Segrate, Italy). ..

    Polymerase Chain Reaction:

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Agarose Gel Electrophoresis:

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands
    Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Image Lab TM software version 5.2.1 (BioRad, Hercules, CA, USA). ..

    Article Title: Altered Capacity for H 2 S Production during the Spontaneous Differentiation of Caco-2 Cells to Colonocytes Due to Reciprocal Regulation of CBS and SELENBP1
    Article Snippet: .. Thereafter, black PbS spots were detected on the agarose gel using a ChemiDoc TM MP analyzer, and pixel density was determined using Image Lab TM software, version 5.2.1 (Bio-Rad Laboratories). ..

    Staining:

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Imaging:

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Article Title: Removal of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli , ST98, in Water for Human Consumption by Black Ceramic Water Filters in Low-Income Ecuadorian Highlands
    Article Snippet: Electrophoresis was programmed at 100 V for 30 min in a Labnet Enduro Gel XL horizontal chamber (Labnet International, Inc., Edison, NJ, USA). .. The agarose gel was visualized on a ChemiDoc TM Imaging Systems photo-documentation system (BioRad, Hercules, CA, USA) by means of Image Lab TM software version 5.2.1 (BioRad, Hercules, CA, USA). ..

    Hood:

    Article Title: Variation in Growth, Morphology, and Fungicide Sensitivity Among Monilinia Species from South Tyrol’s Alpine Orchards
    Article Snippet: For the PCR reactions, 30 μL reaction mixture was used: 1 × Phusion HF Buffer, 0.2 mM dNTPs, 0.4 μM of each primer, 0.02 U μL −1 Phusion TM High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc., Waltham, MA, USA), and 3 μL DNA template. .. The cycling conditions consisted of an initial denaturation at 95 °C for 2 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. PCR products were run on a 1.5% agarose gel, stained with GelRed ® Nucleic Acid Stain (Merck KGaA, Darmstadt, Germany), and viewed on a ChemiDoc TM Imaging System with Image Lab TM Software version 5.2.1 (Universal Hood III, Bio-Rad Laboratories Inc., Hercules, CA, USA). .. Successfully amplified products were sent for Sanger sequencing to LGC Genomics GmbH (Berlin, Germany).

    Membrane:

    Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Three liver tumor organoids were pooled into one, and total RNA was isolated using TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Total RNA was prepared from liver organoids treated with TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).

    Incubation:

    Article Title: Interferon-β Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated overnight at 4 °C with primary antibodies against TRAIL (AF375; R&D Systems, Minneapolis, MA, USA), cleaved caspase-3 (CC3, MAB835; R&D Systems), caspase-3 (pro-C3, MAB707; R&D Systems), and GAPDH (sc47724; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology Danvers, MA, USA, or AP106P; Millipore) for 1 h. After washing thrice with TBST, the bands were visualized using EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and recorded using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Three liver tumor organoids were pooled into one, and total RNA was isolated using TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages
    Article Snippet: The membrane was blocked with 5% skim milk in Tris-HCl-buffered saline containing 0.05% Tween 20 (TBST) for 30 min and incubated with primary antibodies against α-SMA (ab7817 or ab5694; Abcam, Cambridge, UK), COL1A1 (#39952; Cell Signaling Technology, Danvers, MA, USA), CD31 (sc376764; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (sc47724; Santa Cruz Biotechnology) overnight at 4 °C. .. The membrane was washed thrice for 5 min with TBST and incubated with horseradish peroxidase-conjugated secondary antibodies (7074S and 7076S; Cell signaling Technology) for 2 h. After washing thrice with TBST, the bands were treated with an EZ-Western Lumi Pico or Femto (Dogen, Seoul, Republic of Korea) and detected using a ChemiDoc XRS+ System with Image Lab TM software Version 5.2.1 (Bio-Rad Laboratories, Hercules, CA, USA). .. Total RNA was prepared from liver organoids treated with TRIzol reagent (Gibco BRL). cDNA was synthesized from total RNA (1 μg) using a Verso cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA).



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    Effect of polydatin and nicotinamide treatment on complex I and complex IV activities in untreated and treated control (C1) and mutant fibroblasts (P1, P2, P3). The treatment with polydatin and nicotinamide at 10 µM was administered for seven days (+T). ( A ) Complex I activity was measured using the Complex I Enzyme Activity Dipstick Assay Kit from Abcam (ab109720). Complex IV activity was measured using the Complex IV Enzyme Activity Dipstick Assay Kit from Abcam (ab109876). ( B ) Band intensity of complex I activity was obtained using <t>ImageLab</t> software. ( C ) Band intensity of complex IV activity was obtained using ImageLab software. C represents the mean of C1, C2, and C3 cells. Data represent the mean ± SEM of three separate experiments. **** p < 0.0001 between control and GFM1 fibroblasts. #### p < 0.0001 between untreated and treated patient cells. a.u.: arbitrary units.
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    Image Search Results


    Effect of polydatin and nicotinamide treatment on complex I and complex IV activities in untreated and treated control (C1) and mutant fibroblasts (P1, P2, P3). The treatment with polydatin and nicotinamide at 10 µM was administered for seven days (+T). ( A ) Complex I activity was measured using the Complex I Enzyme Activity Dipstick Assay Kit from Abcam (ab109720). Complex IV activity was measured using the Complex IV Enzyme Activity Dipstick Assay Kit from Abcam (ab109876). ( B ) Band intensity of complex I activity was obtained using ImageLab software. ( C ) Band intensity of complex IV activity was obtained using ImageLab software. C represents the mean of C1, C2, and C3 cells. Data represent the mean ± SEM of three separate experiments. **** p < 0.0001 between control and GFM1 fibroblasts. #### p < 0.0001 between untreated and treated patient cells. a.u.: arbitrary units.

    Journal: Biomolecules

    Article Title: Polydatin and Nicotinamide Rescue the Cellular Phenotype of Mitochondrial Diseases by Mitochondrial Unfolded Protein Response (mtUPR) Activation

    doi: 10.3390/biom14050598

    Figure Lengend Snippet: Effect of polydatin and nicotinamide treatment on complex I and complex IV activities in untreated and treated control (C1) and mutant fibroblasts (P1, P2, P3). The treatment with polydatin and nicotinamide at 10 µM was administered for seven days (+T). ( A ) Complex I activity was measured using the Complex I Enzyme Activity Dipstick Assay Kit from Abcam (ab109720). Complex IV activity was measured using the Complex IV Enzyme Activity Dipstick Assay Kit from Abcam (ab109876). ( B ) Band intensity of complex I activity was obtained using ImageLab software. ( C ) Band intensity of complex IV activity was obtained using ImageLab software. C represents the mean of C1, C2, and C3 cells. Data represent the mean ± SEM of three separate experiments. **** p < 0.0001 between control and GFM1 fibroblasts. #### p < 0.0001 between untreated and treated patient cells. a.u.: arbitrary units.

    Article Snippet: The revealed membranes were then analyzed by ImageLab TM software version 5.2.1 (BioRad, Hercules, CA, USA).

    Techniques: Control, Mutagenesis, Activity Assay, Software